MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURE
Routine Culturing of ES Cells
ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS
MITOMYCIN C TREATMENT OF PMEFs
GAMMA IRRADIATION OF PMEF'S
PMEF FEEDER LAYER CONCENTRATION
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
KARYOTYPING ES CELLS
VASECTOMY OF MALE MICE
Normaski Optics
MEDIA FOR EMBRYO CULTURE AND MANIPULATION
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PREPARATION OF MICROINJECTION PIPETTES
EMBRYO MANIPULATION PIPETTES (FLUSHERS ETC)
COLLECTION OF MORULAE and Earlier
Aggregation of ES cells and eight cell stage embryos
BLASTOCYST TRANSFER
Sending sera to testing for mouse pathogens.
SUPEROVULATION OF MICE
FLUSHING OUT OF BLASTOCYSTS
Alizarin Red S (bone) and alcian blue (cartilagestaining of cleared skeletons.
Routine Splitting and freezing of cells
SOFT AGAR ASSAY FOR COLONY FORMATION
VIRUS INFECTION OF FIBROBLASTS
DNA TRANSFECTION OF EUKARYOTIC CELLS USING CALCIUM PHOSPHATE
LIPOFECTAMINE FOR TRANSFORMATION OF EUKARYOTIC CELLS
ELECTROPORATION OF COS-7 CELLS
Growth and transfection of PC12 cells.
CELL CULTURE FROM WHOLE MICE EMBRYO (DAY 10 - 11 PC)
PCR GENOTYPING FROM the EMBRYO YOKE SACK
b-gal staining of eukaryotic cells in vitro
Comments on db-cAMP and forskolin
Comments on Ro 31.8220
IMMUNOFLUORESENCE ON CHAMBER SLIDES
PHOTOMICROGRAPHY
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