Asymmetric sequences are indicated by *.
Single letter code:
R = G or A;
Y = C or T;
W = A or T;
M = A or C;
K = G or T;
S = C or G;
H = A, C or T;
V = A, C or G;
B = C, G or T;
D = A, G or T;
N = G, A, T or C.
Recognition
sequenceEnzyme 3'-protruding end (5'=>3') 1nt 2nt 3nt 4nt 9nt ACGT^ TaiI ACGT ACN^GT TaaI N ACTGG(1/-1)^* BseNI GN ACTGGG(5/4)^* BfiI N ATGCA^T Mph1103I TGCA CACNNN^GTG AdeI NNN ^(0/2)CACTGC* BtsI NN CAGNNN^CTG CaiI NNN CASTG(2/-7)^ TspRI NNCASTGNN ^(0/2)CATCC* BseGI NN CATG^ NlaIII CATG ^(0/2)CATTGC* BseMI NN ^(-1/1)CCAGT* BseNI NC CCANNNNN^NNNNTGG XcmI N CCANNNNN^NTGG BstXI NNNN CCANNNN^NTGG Van91I NNN ^(4/5)CCCAGT* BfiI N CCGC^GG Cfr42I GC CCNNNNN^NNGG BseLI NNN CCTC(7/6)^* MnlI N CCTGCA^GG SdaI TGCA CGAT^CG PvuI AT CGRY^CG Bsh1285I RY CTCAG(10/8)^* BseMII NN ^(14/16)CTCCAG* GsuI NN ^(8/10)CTCCTC* BseRI NN CTGAAG(16/14)^* Eco57I NN CTGRAG(16/14)^* Eco57MI NN ^(8/10)CTGAG* BseMII NN ^(14/16)CTGCAC* BsgI NN CTGCA^G PstI TGCA CTGGAG(16/14)^* GsuI NN ^(14/16)CTTCAG* Eco57I NN
Recognition
sequenceEnzyme 3'-protruding end (5'=>3') 1nt 2nt 3nt 4nt 9nt GAAGA(8/7)^* MboII N GAATGC(1/-1)^* Mva1269I CN GACGT^C AatII ACGT GACNNN^NNGTC Eam1105I 其他網友還關注過
- 內切酶列表：Enzymes Generating Blunt Ends
- 內切酶列表：Enzymes Generating Blunt Ends
- 內切酶列表：Enzymes Generating 3'-protruding Ends
- 內切酶列表：Enzymes Generating 5'-protruding Ends
- Generating stable cell lines in HEK293
- Generating stable cell lines in HEK293
- DNA的酶學操作
- RACE(rapid-amplification of cDNA ends)技術-1
- RACE(rapid-amplification of cDNA ends)技術-2
- Cloning of small RNAs with 5’ phosphate and 3’ OH ends-2
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